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Image Search Results
Journal: British journal of haematology
Article Title: Phage display generation of a novel human anti-CD1A monoclonal antibody with potent cytolytic activity
doi: 10.1111/bjh.12033
Figure Lengend Snippet: Reactivity and specificity of the human anti-CD1A monoclonal antibodies (mAbs) on different CD1 antigens expressed in the C1R and B16 cell lines. C1R cells expressing either CD1A, CD1B, CD1C or CD1D or no CD1 antigens (termed C1R mock) as well as B16 cells transfected with a viral expression vector containing the cDNA encoding CD1A or empty vector were stained with either CR2113, CR2114, CR2115, CR2116, CR2117 or CR2118 mAbs at 1, 3, and 10 μg/ml. Data for 10 μg mAb/ml are shown and representative of all three doses. Mouse IgG1, κ-FITC was used as a secondary antibody. Cells were analysed by flow cytometry using Kolmogorov-Smirnov statistics to quantitate binding as described in Methods. Specificity was determined by the level of binding to cells expressing specific CD1 isoforms compared to background, isotype control antibody staining. Statistical significance was determined by t-test (unpaired, two-tailed) analysis when comparing the binding of specific monoclonal antibodies to background staining assessed by antibody isotype control staining on cells expressing specific CD1 isoforms. Error bars represent standard deviations. CR2113, CR2114, CR2117 and CR2118 mAbs showed significant binding to CD1A expressing cells with CR2113 having the strongest specific binding characteristics. CR2114 and CR2117 also showed some affinity for CD1C. Legend: ***: p< 0.001, **: p< 0.01, *: p<0.05, ns: p>0.05
Article Snippet: Isotype control antibodies included mAb CR2027, IgG1, GBS III-biotin-labelled
Techniques: Expressing, Transfection, Plasmid Preparation, Staining, Flow Cytometry, Binding Assay, Two Tailed Test